C0773

Sigma

 

Collagenase from Clostridium histolyticum

high purity, purified by chromatography, Type VII, 4-12 FALGPA units/mg solid, lyophilized powder, 1,000-3,000 CDU/mg solid (CDU = collagen digestion units)

Synonym:Clostridiopeptidase A
CAS Number:9001-12-1
Enzyme Commission (EC) Number:3.4.24.3   ( BRENDA | IUBMB )
EC Number:232-582-9
MDL number:MFCD00130830

Description

Biochem/physiol ActionsTypically used with a neutral protease for tissue disruption.
Other NotesView more information on collagenase and cell dissociation at www.sigma-aldrich.com/enzymeexplorer.
Unit DefinitionOne collagen digestion unit liberates peptides from collagen equivalent in ninhydrin color to 1.0 μmole of leucine in 5 hr at pH 7.4 at 37 °C in the presence of calcium ions.
One FALGPA hydrolysis unit hydrolyzes 1.0 μmole of furylacryloyl-Leu-Gly-Pro-Ala per min at pH 7.5 at 25 °C in the presence of calcium ions.
One neutral protease unit hydrolyzes casein to produce color equivalent to 1.0 μmole tyrosine per 5 hr at pH 7.5 at 37 °C.
One clostripain unit hydrolyzes 1.0 μmole of BAEE per min at pH 7.6 at 25 °C in the presence of DTT.
Physical formLyophilized powder containing calcium chloride

Properties

typeType VII
formlyophilized powder
purified bychromatography
compositionProtein, ≥90% biuret
foreign activityneutral protease and clostripain <1 unit/mg protein
storage temp.−20°C

Safety

Hazard CodesXn
Risk Statements36/37/38-42
Safety Statements22-24-26-36/37
WGK Germany1

Related Products

SubstrateF5135, N-[3-(2-Furyl)acryloyl]-Leu-Gly-Pro-Ala
 C0627, Z-Gly-Pro-Leu-Gly-Pro
InhibitorM3531, (2R)-2-Mercaptomethyl-4-methylpentanoyl-β-(2-naphthyl)-Ala-Ala Amide

References

MerckMerck 13,2505